Internal Contamination (USUHS)
Abstract
Internal Contamination (USU): Radioactive material can enter the body by a variety of pathways including ingestion, inhalation, and wound contamination. While some internalized isotopes will be naturally eliminated from the body, many others are not. They remain immobile or are transported and deposited to other organs where they continually irradiate the surrounding tissue. This chronic internal radiation exposure can cause unrepairable cellular damage eventually leading to death. This Program uses innovative approaches to address this pressing health concern. FY21 Accomplishments: (1)Determined a chemical synthesis route containing a magnetic core. (2)Tested the ability of non-magnetic dendrimers to bind uranium and cesium. (3)Completed the synthesis of uranium and cesium-templated dendrimers for high-specific metal binding imprinted polymers. (4)Completed the preparation of dendrimers with standard metal chelators attached to their terminal ends. (5)Assessed the ability of dendrimer containing metal chelators using a novel in vitro system. (6)Initiated cytotoxicity assessments of the novel chemically synthesized imprinted polymers. (7)Determined the efficacy of molecular imprinted polymers on reducing the body burden of internalized radionuclides using the novel in vitro system. (8)Received IACUC approval. (9)Animal specimens were submitted for histopathological evaluation and are being evaluated by a board certified pathologist. (10)Sternal sections were evaluated for Megakaryocytes, indicative of proliferation. (11)Bone marrow was assayed for colony forming units, indicative of proliferation effects and complete blood cell counts were analyzed. (12)Fecal pellets were collected from male and female C57BL/6 mice one and six month’s post-TBI were submitted to WRAIR for 16S microbiome sequencing. (13)Serum samples were collected and submitted to Georgetown University for metabolic and lipidomics analysis. (14)Fabricated of gut organ-on-chip model and quality control evaluation. (15)Identified and evaluated small molecules for gut organ-on-chip model. (16)In 2019/2021, five manuscripts were published.
Document Details
- Document Type
- Accomplishment
- Publication Date
- Oct 01, 2023
- Source ID
- 0f35b5ec041efd5ddd04cee88ee4709a