A patient‐derived explant (PDE) model of hormone‐dependent cancer
Abstract
Breast and prostate cancer research to date has largely been predicated on the use of cell lines in vitro or in vivo. These limitations have led to the development of more clinically relevant models, such as organoids or murine xenografts that utilize patient‐derived material; however, issues related to low take rate, long duration of establishment, and the associated costs constrain use of these models. This study demonstrates that ex vivo culture of freshly resected breast and prostate tumor specimens obtained from surgery, termed patient‐derived explants (PDEs), provides a high‐throughput and cost‐effective model that retains the native tissue architecture, microenvironment, cell viability, and key oncogenic drivers. The PDE model provides a unique approach for direct evaluation of drug responses on an individual patient's tumor, which is amenable to analysis using contemporary genomic technologies. The ability to rapidly evaluate drug efficacy in patient‐derived material has high potential to facilitate implementation of personalized medicine approaches.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 16, 2018
- Source ID
- 10.1002/1878-0261.12354
Entities
People
- Claus G. Roehrborn
- Clive E. Hoffmann
- Ganesh V Raj
- Hisham Mohammed
- Jason S. Carroll
- Jessica L. Robinson
- Karen E. Knudsen
- Leonard G. Gomella
- Lisa M Butler
- Margaret M. Centenera
- Matthew J. Schiewer
- Natalie K. Ryan
- Payal Kapur
- Peter D. Sutherland
- Preethi Ravindranathan
- Shalini Jindal
- Shihong Ma
- Stephen N. Birrell
- Theresa E Hickey
- Wayne D Tilley
Organizations
- Adelaide Medical School
- Australian Research Council
- Cancer Australia
- Cancer Research UK
- European Molecular Biology Organization
- Movember Foundation
- National Breast Cancer Foundation
- National Health and Medical Research Council
- National Institutes of Health
- Oregon Health & Science University
- Prostate Cancer Foundation
- Prostate Cancer Foundation of Australia
- Royal Adelaide Hospital
- South Australian Health and Medical Research Institute
- Susan G. Komen for the Cure
- Thomas Jefferson University
- United States Department of Defense
- University of Adelaide
- University of Cambridge
- University of Texas at Austin