Rabbit M1 and M2 macrophages can be induced by human recombinant GM‐CSF and M‐CSF
Abstract
Macrophages can change their phenotype in response to environmental cues. Polarized macrophages are broadly classified into two groups: classical activated M1 and alternative activated M2. Characterization of human macrophages has been widely studied, but polarized macrophages in rabbits have not been characterized. We characterized rabbit macrophages that were polarized using human recombinant GM‐CSF and M‐CSF. GM‐CSF‐treated macrophages had higher mRNA expression of proinflammatory cytokines (M1 phenotype) than did the M‐CSF‐treated counterpart. By contrast, high levels of TGF‐β and IL‐10 expression (M2 phenotype) were found in M‐CSF‐treated macrophages. The present study may be useful to understand roles of polarized macrophages in rabbit disease models.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 15, 2016
- Source ID
- 10.1002/2211-5463.12101
Entities
People
- Kai‐poon Leung
- Kazuyoshi Yamane
Organizations
- Naval Medical Research Center
- Osaka Dental University
- United States Army