Membrane Protein Insertion into and Compatibility with Biomimetic Membranes
Abstract
Membrane protein and membrane protein–mimic functionalized materials are rapidly gaining interest across a wide range of applications, including drug screening, DNA sequencing, drug delivery, sensors, water desalination, and bioelectronics. In these applications, material performance is highly dependent on activity‐per‐protein and protein packing density in bilayer and bilayer‐like structures collectively known as biomimetic membranes. However, a clear understanding of, and accurate tools to study these properties of biomimetic membranes does not exist. This paper presents methods to evaluate membrane protein compatibility with biomimetic membrane materials. The methods utilized provide average single protein activity, and for the first time, provide experimentally quantifiable measures of the chemical and physical compatibility between proteins (and their mimics) and membrane materials. Water transport proteins, rhodopsins, and artificial water channels are reconstituted into the full range of current biomimetic membrane matrices to evaluate the proposed platform. Compatibility measurement results show that both biological and artificial water channels tested largely preserve their single protein water transport rates in biomimetic membranes, while their reconstitution density is variable, leading to different overall membrane permeabilities. It is also shown that membrane protein insertion efficiency inversely correlates with both chemical and physical hydrophobicity mismatch between membrane protein and the membrane matrix.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 16, 2017
- Source ID
- 10.1002/adbi.201700053
Entities
People
- A. Benjamin Schantz
- Eduardo Barbieri
- Guillermo C. Bazan
- Hasin Feroz
- Hengjing Yan
- Joseph F. Hall
- Manish Kumar
- Mariusz Grzelakowski
- Mustafa Erbakan
- Patrick Saboe
- Peter C Butler
- Samantha R McCuskey
- Tingwei Ren
- Yuexiao Shen
Organizations
- Army Research Office
- Bozok University
- Federal University of Rio de Janeiro
- National Science Foundation
- Pennsylvania State University
- University of California
- University of California, Santa Barbara