Plasmon‐Enhanced Biosensing for Multiplexed Profiling of Extracellular Vesicles
Abstract
Extracellular vesicles (EVs)—nanoscale phospholipid vesicles secreted by cells—present new opportunities for molecular diagnosis from non‐invasive liquid biopsies. Single EV protein analysis can be extremely valuable in studying EVs as circulating cancer biomarkers, but it is technically challenging due to weak detection signals associated with limited amounts of epitopes and small surface areas for antibody labeling. Here, a new, simple method that enables multiplexed analyses of EV markers with improved sensitivities is reported. Specifically, plasmon‐enhanced fluorescence detection is implemented that amplifies fluorescence signals using surface plasmon resonances excited by periodic gold nanohole structures. It is shown that fluorescence signals in multiple channels are amplified by one order of magnitude, and both transmembrane and intravesicular markers can be detected at the single EV level. This approach can offer additional insight into understanding subtypes, heterogeneity, and production dynamics of EVs during disease development and progression.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 19, 2020
- Source ID
- 10.1002/adbi.202000003
Entities
People
- Andreas Wegemann
- Hakho Lee
- Hyungsoon Im
- Jae‐sang Hong
- Jouha Min
- Koushik Murlidharan
- Pike See Cheah
- Ralph Weissleder
- Taehwang Son
Organizations
- Harvard Medical School
- Massachusetts General Hospital
- National Cancer Institute
- National Institute on Aging
- Technical University of Munich
- United States Department of Defense