Rapid Self‐Assembly of Macroscale Tissue Constructs at Biphasic Aqueous Interfaces
Abstract
An entirely new approach to tissue engineering is presented that uses the interfacial forces between aqueous solutions of phase‐separating polymers to confine cells and promote their assembly into interconnected, macroscopic tissue constructs. This simple and inexpensive general procedure creates free‐standing, centimeter‐scale constructs from cell suspensions at the interface between poly(ethylene glycol) and dextran aqueous two‐phase systems in as little as 2 h. Using this method, skin constructs are produced that integrate with decellularized dermal matrices, on which they differentiate and stratify into skin equivalents. It is demonstrated that the constructs produced by this method have appropriate integrity and mechanical properties for use as in vitro tissue models.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 06, 2015
- Source ID
- 10.1002/adfm.201403825
Entities
People
- Brendan M. Leung
- Eve L. Bingham
- Hady T. Sarhan
- Jack D. Wang
- John P. Frampton
- Mohamed E. H. El‐sayed
- Sasha Cai Lesher‐perez
- Shuichi Takayama
- Stephen E. Feinberg
Organizations
- Dalhousie University
- Defense Threat Reduction Agency
- University of Michigan