Gold Nanocomposite Bioink for Printing 3D Cardiac Constructs
Abstract
Bioprinting is the most convenient microfabrication method to create biomimetic three‐dimensional (3D) cardiac tissue constructs, that can be used to regenerate damaged tissue and provide platforms for drug screening. However, existing bioinks, which are usually composed of polymeric biomaterials, are poorly conductive and delay efficient electrical coupling between adjacent cardiac cells. To solve this problem, a gold nanorod (GNR)‐incorporated gelatin methacryloyl (GelMA)‐based bioink is developed for printing 3D functional cardiac tissue constructs. The GNR concentration is adjusted to create a proper microenvironment for the spreading and organization of cardiac cells. At optimized concentrations of GNR, the nanocomposite bioink has a low viscosity, similar to pristine inks, which allows for the easy integration of cells at high densities. As a result, rapid deposition of cell‐laden fibers at a high resolution is possible, while reducing shear stress on the encapsulated cells. In the printed GNR constructs, cardiac cells show improved cell adhesion and organization when compared to the constructs without GNRs. Furthermore, the incorporated GNRs bridge the electrically resistant pore walls of polymers, improve the cell‐to‐cell coupling, and promote synchronized contraction of the bioprinted constructs. Given its advantageous properties, this gold nanocomposite bioink may find wide application in cardiac tissue engineering.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 17, 2017
- Source ID
- 10.1002/adfm.201605352
Entities
People
- Ali Khademhosseini
- Ali Tamayol
- Amir Nasajpour
- Jeroen Leijten
- Kai Zhu
- Mohammad Asif Hussain
- Ning Hu
- Serena Mandla
- Su Ryon Shin
- Tim Van Kempen
- Vidhya Ponraj
- Xiao Liu
- Yi‐chen Li
- Yi‐dong Lin
- Yu Shrike Zhang
Organizations
- Defense Threat Reduction Agency
- Fudan University
- Harvard Medical School
- Harvard University
- King Abdulaziz University
- Konkuk University
- National Cancer Institute
- National Institutes of Health
- Office of Naval Research
- Shanghai Education Development Foundation
- Shanghai Municipal Education Commission
- University of Twente