Gram‐Typing Using Conjugated Oligoelectrolytes
Abstract
It is shown that Gram‐type differentiation, an essential tool in microbiology, can now be easily accomplished by using the chain‐elongated conjugated oligoelectrolyte COE‐S6. Specifically, COE‐S6 can be used as a fluorescent membrane probe that distinguishes between Gram‐negative and Gram‐positive bacteria in a single step. Confocal microscopy of labeled microorganisms and differential scanning calorimetry with phospholipid‐ and lipopolysaccharide‐containing model membranes suggest that lipopolysaccharides impede COE‐S6 membrane intercalation in Gram‐negative bacteria. Both Gram‐types within a mixture are discretely labeled by counterstaining COE‐S6 with the nonspecific and lipophilic membrane probe FM 4–64. This single‐step procedure allows for the in situ visualization of individual Gram‐types in complex polymicrobial biofilms. Given that COE‐S6 fluorescence intensity increases considerably post intercalation, it can be used for the detection of Gram‐positive bacteria by the naked eye. COE‐S6 does not inhibit bacterial growth and is simple to use, making it a promising membrane‐specific fluorescent probe.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 19, 2020
- Source ID
- 10.1002/adfm.202004068
Entities
People
- Alex S. Moreland
- Bo Liedberg
- Cheng Zhou
- Geraldine W. N. Chia
- Guillermo C. Bazan
- James C. S. Ho
- Jamie Hinks
- Lin Ruan
- Staffan Kjelleberg
Organizations
- Ministry of Education
- Nanyang Technological University
- National University of Singapore
- The Institute for Collaborative Biotechnologies
- University of California, Santa Barbara
- Yusuf Hamied Department of Chemistry