Electrical Differentiation of Mesenchymal Stem Cells into Schwann‐Cell‐Like Phenotypes Using Inkjet‐Printed Graphene Circuits
Abstract
Graphene‐based materials (GBMs) have displayed tremendous promise for use as neurointerfacial substrates as they enable favorable adhesion, growth, proliferation, spreading, and migration of immobilized cells. This study reports the first case of the differentiation of mesenchymal stem cells (MSCs) into Schwann cell (SC)‐like phenotypes through the application of electrical stimuli from a graphene‐based electrode. Electrical differentiation of MSCs into SC‐like phenotypes is carried out on a flexible, inkjet‐printed graphene interdigitated electrode (IDE) circuit that is made highly conductive (sheet resistance −1 of nerve growth factor (NGF) secretion vs. 75% and ≈55 ng mL−1 for chemically treated MSCs (ctMSCs)]. These results help pave the way for in vivo peripheral nerve regeneration where the flexible graphene electrodes could conform to the injury site and provide intimate electrical simulation for nerve cell regrowth.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 20, 2017
- Source ID
- 10.1002/adhm.201601087
Entities
People
- Allison A. Cargill
- Donald Sakaguchi
- John A. Hondred
- Jonathan Claussen
- Matthew T. Lentner
- Metin Uz
- Shaowei Ding
- Suprem R. Das
- Surya Mallapragada
Organizations
- Ames National Laboratory
- Iowa State University
- Roy J. Carver Charitable Trust
- United States Army Medical Research and Development Command