3D Jet Writing: Functional Microtissues Based on Tessellated Scaffold Architectures
Abstract
The advent of adaptive manufacturing techniques supports the vision of cell‐instructive materials that mimic biological tissues. 3D jet writing, a modified electrospinning process reported herein, yields 3D structures with unprecedented precision and resolution offering customizable pore geometries and scalability to over tens of centimeters. These scaffolds support the 3D expansion and differentiation of human mesenchymal stem cells in vitro. Implantation of these constructs leads to the healing of critical bone defects in vivo without exogenous growth factors. When applied as a metastatic target site in mice, circulating cancer cells home in to the osteogenic environment simulated on 3D jet writing scaffolds, despite implantation in an anatomically abnormal site. Through 3D jet writing, the formation of tessellated microtissues is demonstrated, which serve as a versatile 3D cell culture platform in a range of biomedical applications including regenerative medicine, cancer biology, and stem cell biotechnology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 27, 2018
- Source ID
- 10.1002/adma.201707196
Entities
People
- Clark B. Teeple
- Gary D. Luker
- Henry R. Haley
- Hongli Sun
- Jacob H. Jordahl
- Joerg Lahann
- Kyung Jin Lee
- Luis Solorio
- Paul H. Krebsbach
- Stacy Ramcharan
- Thomas W. Eyster
Organizations
- National Center for Research Resources
- National Institutes of Health
- National Science Foundation
- United States Department of Defense