Nucleic‐Acid Structures as Intracellular Probes for Live Cells
Abstract
The chemical composition of cells at the molecular level determines their growth, differentiation, structure, and function. Probing this composition is powerful because it provides invaluable insight into chemical processes inside cells and in certain cases allows disease diagnosis based on molecular profiles. However, many techniques analyze fixed cells or lysates of bulk populations, in which information about dynamics and cellular heterogeneity is lost. Recently, nucleic‐acid‐based probes have emerged as a promising platform for the detection of a wide variety of intracellular analytes in live cells with single‐cell resolution. Recent advances in this field are described and common strategies for probe design, types of targets that can be identified, current limitations, and future directions are discussed.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 04, 2019
- Source ID
- 10.1002/adma.201901743
Entities
People
- Chad Mirkin
- Devleena Samanta
- Sasha B Ebrahimi
Organizations
- Air Force Office of Scientific Research
- Air Force Research Laboratory
- National Cancer Institute
- National Institutes of Health
- Northwestern University
- Office of Naval Research