Layer‐by‐Layer Engineered All‐Liquid Microfluidic Chips for Enzyme Immobilization
Abstract
Enzyme immobilization in the confines of microfluidic chips, that promote enzyme activity and stability, has become a powerful strategy to enhance biocatalysis and biomass conversion. Here, based on a newly developed all‐liquid microfluidic chip, fabricated by the interfacial assembly of nanoparticle surfactants (NPSs) in a biphasic system, a layer‐by‐layer assembly strategy to generate polysaccharide multilayers on the surface of a microchannel, greatly enhancing the mechanical properties of the microchannel and offering a biocompatible microenvironment for enzyme immobilization, is presented. Using horseradish peroxidase and glucose oxidase as model enzymes, all‐liquid microfluidic enzymatic and cascade reactors have been constructed and the crucial role of polysaccharide multilayers on enhancing the enzyme loading and catalytic efficiency is demonstrated.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 22, 2021
- Source ID
- 10.1002/adma.202105386
Entities
People
- Shaowei Shi
- Tan Liu
- Thomas Paul Russell
- Yang Yang
- Yixuan Yin
Organizations
- Army Research Office
- Beijing Municipal Natural Science Foundation
- Beijing University of Chemical Technology
- Lawrence Berkeley National Laboratory
- National Natural Science Foundation of China
- Office of Basic Energy Sciences
- Office of Science
- United States Department of Energy
- University of Massachusetts