Multiplexed Ultrasensitive Sample‐to‐Answer RT‐LAMP Chip for the Identification of SARS‐CoV‐2 and Influenza Viruses
Abstract
Prompt on‐site diagnosis of SARS‐CoV‐2 with other respiratory infections will have minimized the global impact of the COVID‐19 pandemic through rapid, effective management. However, no such multiplex point‐of‐care (POC) chip has satisfied a suitable sensitivity of gold‐standard nucleic acid amplification tests (NAATs). Here, a rapid multiplexed ultrasensitive sample‐to‐answer loop‐mediated isothermal amplification (MUSAL) chip operated by simple LED‐driven photothermal amplification to detect six targets from single‐swab sampling is presented. First, the MUSAL chip allows ultrafast on‐chip sample preparation with ≈500‐fold preconcentration at a rate of 1.2 mL min−1. Second, the chip enables contamination‐free amplification using autonomous target elution into on‐chip reagents by photothermal activation. Finally, the chip accomplishes multiplexed on‐chip diagnostics of SARS‐CoV‐2 and influenza viruses with a limit of detection (LoD) of 0.5 copies µL−1. The rapid, ultrasensitive, cost‐effective sample‐to‐answer chip with a multiplex capability will allow timely management of various pandemics situations that may be faced shortly.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 26, 2023
- Source ID
- 10.1002/adma.202207138
Entities
People
- Luke P Lee
- Minsun Song
- SoonGweon Hong
Organizations
- Air Force Office of Scientific Research
- Brigham and Women's Hospital
- Harvard University
- Sungkyunkwan University
- University of California, Berkeley