Spontaneous Reconstitution of Functional Transmembrane Proteins During Bioorthogonal Phospholipid Membrane Synthesis
Abstract
Transmembrane proteins are critical for signaling, transport, and metabolism, yet their reconstitution in synthetic membranes is often challenging. Non‐enzymatic and chemoselective methods to generate phospholipid membranes in situ would be powerful tools for the incorporation of membrane proteins. Herein, the spontaneous reconstitution of functional integral membrane proteins during the de novo synthesis of biomimetic phospholipid bilayers is described. The approach takes advantage of bioorthogonal coupling reactions to generate proteoliposomes from micelle‐solubilized proteins. This method was successfully used to reconstitute three different transmembrane proteins into synthetic membranes. This is the first example of the use of non‐enzymatic chemical synthesis of phospholipids to prepare proteoliposomes.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 28, 2015
- Source ID
- 10.1002/ange.201504339
Entities
People
- Christian M. Cole
- Jerry Yang
- Michael D. Hardy
- Neal Devaraj
- Roberto J. Brea
- Young Hun Kim
Organizations
- Air Force Office of Scientific Research
- Army Research Office
- National Institutes of Health