Whole‐Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample
Abstract
Metagenomic studies suggest that only a small fraction of the viruses that exist in nature have been identified and studied. Characterization of unknown viral genomes is hindered by the many genomes populating any virus sample. A new method is reported that integrates drop‐based microfluidics and computational analysis to enable the purification of any single viral species from a complex mixed virus sample and the retrieval of complete genome sequences. By using this platform, the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater was retrieved with greater than 96 % sequence coverage and more than 99.8 % sequence identity. This method holds great potential for virus discovery since it allows enrichment and sequencing of previously undescribed viruses as well as known viruses.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 28, 2015
- Source ID
- 10.1002/ange.201507047
Entities
People
- Assaf Rotem
- David A Weitz
- Hee‐sun Han
- James M. Pipas
- Martial Carbonnaux
- Paul G. Cantalupo
- Shelley K. Cockrell
Organizations
- Defense Advanced Research Projects Agency