Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold
Abstract
Artificial multi‐enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD+ substrate channeling by specifically shifting NAD+ between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 09, 2016
- Source ID
- 10.1002/ange.201603183
Entities
People
- Chaoyong Yang
- Fei Zhang
- Guoliang Ke
- Hao Yan
- Minghui Liu
- Shaun Wootten
- Shuoxing Jiang
- Xiaodong Qi
- Yan Y Liu
- Yuhe Renee Yang
- Zhi Zhu
Organizations
- Biodesign Institute
- National Science Foundation
- Xiamen University