A Biomimetic Phosphatidylcholine‐Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer‐Based Sensors
Abstract
The real‐time monitoring of specific analytes in situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash‐free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer‐based (E‐AB) sensors are promising candidates to fill this role. E‐AB sensors suffer, however, from often‐severe baseline drift when deployed in undiluted whole blood either in vitro or in vivo. We demonstrate that cell‐membrane‐mimicking phosphatidylcholine (PC)‐terminated monolayers improve the performance of E‐AB sensors, reducing the baseline drift from around 70 % to just a few percent after several hours in flowing whole blood in vitro. With this improvement comes the ability to deploy E‐AB sensors directly in situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift‐correction algorithms.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 28, 2017
- Source ID
- 10.1002/ange.201700748
Entities
People
- Christina Shin
- Claire H. Tran
- Hui Li
- Jacob Somerson
- Kevin W Plaxco
- Netzahualcóyotl Arroyo-Currás
- Philip A. Vieira
- Philippe Dauphin‐ducharme
- Shaoguang Li
- Tod E. Kippin
Organizations
- Foundation for the National Institutes of Health
- Swiss National Science Foundation
- United States Army Research Laboratory
- University of California
- University of California, Santa Barbara
- W. M. Keck Foundation