Photon‐Induced Near‐Field Electron Microscopy of Eukaryotic Cells
Abstract
Photon‐induced near‐field electron microscopy (PINEM) is a technique to produce and then image evanescent electromagnetic fields on the surfaces of nanostructures. Most previous applications of PINEM have imaged surface plasmon‐polariton waves on conducting nanomaterials. Here, the application of PINEM on whole human cancer cells and membrane vesicles isolated from them is reported. We show that photons induce time‐, orientation‐, and polarization‐dependent evanescent fields on the surfaces of A431 cancer cells and isolated membrane vesicles. Furthermore, the addition of a ligand to the major surface receptor on these cells and vesicles (epidermal growth factor receptor, EGFR) reduces the intensity of these fields in both preparations. We propose that in the absence of plasmon waves in biological samples, these evanescent fields reflect the changes in EGFR kinase domain polarization upon ligand binding.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 10, 2017
- Source ID
- 10.1002/ange.201706120
Entities
People
- Ahmed Zewail
- Bolin Liao
- Byung‐kuk Yoo
- David Baltimore
- Devdoot Majumdar
- Grant J. Jensen
- Jau Tang
- Mohammed Kaplan
- Tony E. Karam
Organizations
- Air Force Office of Scientific Research
- California Institute of Technology
- Howard Hughes Medical Institute
- National Science Foundation