Super‐resolution Geometric Barcoding for Multiplexed miRNA Profiling
Abstract
MicroRNA (miRNA) expression profiles hold promise as biomarkers for diagnostics and prognosis of complex diseases. Herein, we report a super‐resolution fluorescence imaging‐based digital profiling method for specific, sensitive, and multiplexed detection of miRNAs. In particular, we applied the DNA‐PAINT (point accumulation for imaging in nanoscale topography) method to implement a super‐resolution geometric barcoding scheme for multiplexed single‐molecule miRNA capture and digital counting. Using synthetic DNA nanostructures as a programmable miRNA capture nano‐array, we demonstrated high‐specificity (single nucleotide mismatch discrimination), multiplexed (8‐plex, 2 panels), and sensitive measurements on synthetic miRNA samples, as well as applied one 8‐plex panel to measure endogenous miRNAs levels in total RNA extract from HeLa cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 04, 2018
- Source ID
- 10.1002/ange.201807956
Entities
People
- Mingjie Dai
- Peng Yin
- Weidong Xu
Organizations
- Harvard Medical School
- Harvard University
- National Institutes of Health
- National Science Foundation
- Office of Naval Research