Modulating Self‐Assembly of DNA Crystals with Rationally Designed Agents
Abstract
This manuscript reports a strategy for controlling the crystallization kinetics and improving the quality of engineered self‐assembled 3D DNA crystals. Growing large, high‐quality biomacromolecule crystals is critically important for determining the 3D structures of biomacromolecules. It often presents a great challenge to structural biologists. Herein, we introduce a rationally designed agent to modulate the crystallization process. Under such conditions, fewer, but larger, crystals that yield diffraction patterns of modestly higher resolution are produced compared with the crystals from conditions without the modulating agent. We attribute the improvement to a smaller number of nuclei and slow growth rate of crystallization. This strategy is expected to be generally applicable for crystallization of other biomacromolecules.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 08, 2018
- Source ID
- 10.1002/ange.201809757
Entities
People
- Chengde Mao
- Jiemin Zhao
- Nadrian C. Seeman
- Ruojie Sha
- Yong Wang
- Yue Zhao
- Zhe Li
Organizations
- Gordon and Betty Moore Foundation
- Nanchang University
- National Science Foundation
- New York University
- Office of Naval Research
- Purdue University
- United States Department of Energy