Inhibition of Pathogen Adhesion by Bacterial Outer Membrane‐Coated Nanoparticles

Abstract

Anti‐adhesion therapies interfere with the bacterial adhesion to the host and thus avoid direct disruption of bacterial cycles for killing, which may alleviate resistance development. Herein, an anti‐adhesion nanomedicine platform is made by wrapping synthetic polymeric cores with bacterial outer membranes. The resulting bacterium‐mimicking nanoparticles (denoted “OM‐NPs”) compete with source bacteria for binding to the host. The “top‐down” fabrication of OM‐NPs avoids the identification of the adhesins and bypasses the design of agonists targeting these adhesins. In this study, OM‐NPs are made with the membrane of Helicobacter pylori and shown to bind with gastric epithelial cells (AGS cells). Treatment of AGS cells with OM‐NPs reduces H. pylori adhesion and such anti‐adhesion efficacy is dependent on OM‐NP concentration and its dosing sequence.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jul 03, 2019
Source ID
10.1002/ange.201906280

Entities

People

  • Christopher Lo
  • Jia Zhuang
  • Liangfang Zhang
  • Marygorret Obonyo
  • Pavimol Angsantikul
  • Qiangzhe Zhang
  • Ronnie H Fang
  • Weiwei Gao
  • Xiaoli Wei
  • Yijie Chen
  • Yue Zhang
  • Zhidong Zhou

Organizations

  • Defense Threat Reduction Agency
  • University of California, San Diego

Tags

Fields of Study

  • Biology

Readers

  • Forest Ecology
  • Microbial Pathology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech