Rational Design of pH‐Responsive DNA Motifs with General Sequence Compatibility
Abstract
Reconfigurable molecular events are key to molecular machines. In response to external cues, molecular machines rearrange/change their structures to perform certain functions. Such machines exist in nature, for example cell surface receptors, and have been artificially engineered. To be able to build sophisticated and efficient molecular machines for an increasing range of applications, constant efforts have been devoted to developing new mechanisms of controllable structural reconfiguration. Herein, we report a general design principle for pH‐responsive DNA motifs for general DNA sequences (not limited to triplex or i‐motif forming sequences). We have thoroughly characterized such DNA motifs by polyacrylamide gel electrophoresis (PAGE) and fluorescence spectroscopy and demonstrated their applications in dynamic DNA nanotechnology. We expect that it will greatly facilitate the development of DNA nanomachines, biosensing/bioimaging, drug delivery, etc.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 01, 2019
- Source ID
- 10.1002/ange.201906972
Entities
People
- Cheng Zhi Huang
- Chengde Mao
- Gaohui Wei
- Hua Zuo
- Jie Zeng
- Liang Fang
- Linlin Tang
- Renjie Zhan
- Wenhao Fu
- Xuemei Liu
Organizations
- Office of Naval Research
- Purdue University
- Southwest University