Consequences of Noncovalent Interfacial Contacts between Nanoparticles and Giant Vesicles
Abstract
Biological membrane fluidity enables shape reform upon a functionally complementary encounter between a biomolecule and a synthetic nanoparticle. Nanomedicine uses ligand‐cell surface receptor affinities for nanoparticle targeting. However, due to toxicity concerns, the nature of the nanoparticle–biomembrane interaction needs exploration to realize ligand surface density and receptor–ligand interaction effects on subsequent uptake/other interaction outcomes. In this study, giant unilamellar vesicles (GUVs) were surface‐immobilized with a hCAII model receptor to study nanoparticle–complementary surface ligand interactions. We find interaction strength impacts morphological outcomes, namely, inter‐GUV adhesion, lysis, and GUV swallowing. Nanoparticle size, receptor/ligand surface densities, overall receptor/ligand concentrations and ratios affect the strength of adhesion, and thus the outcome. Interaction energy effects on GUV morphological outcomes offer fundamental insights into the design of abiological materials for interacting with biological materials.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 25, 2022
- Source ID
- 10.1002/ange.202208616
Entities
People
- Ann Fernandez
- Anthony D Dinsmore
- Francesca Anson
- Jithu Krishna
- Sankaran Thayumanavan
Organizations
- Army Research Office
- University of Massachusetts
- University of Massachusetts Amherst