Programmable 3D Hexagonal Geometry of DNA Tensegrity Triangles
Abstract
Non‐canonical interactions in DNA remain under‐explored in DNA nanotechnology. Recently, many structures with non‐canonical motifs have been discovered, notably a hexagonal arrangement of typically rhombohedral DNA tensegrity triangles that forms through non‐canonical sticky end interactions. Here, we find a series of mechanisms to program a hexagonal arrangement using: the sticky end sequence; triangle edge torsional stress; and crystallization condition. We showcase cross‐talking between Watson–Crick and non‐canonical sticky ends in which the ratio between the two dictates segregation by crystal forms or combination into composite crystals. Finally, we develop a method for reconfiguring the long‐range geometry of formed crystals from rhombohedral to hexagonal and vice versa. These data demonstrate fine control over non‐canonical motifs and their topological self‐assembly. This will vastly increase the programmability, functionality, and versatility of rationally designed DNA constructs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 09, 2023
- Source ID
- 10.1002/ange.202213451
Entities
People
- Bena Yang
- Brandon Lu
- Chengde Mao
- Cuizheng Zhang
- Karol Woloszyn
- Nadrian C. Seeman
- Ruojie Sha
- Simon Vecchioni
- Yoel P. Ohayon
Organizations
- National Science Foundation
- New York University
- Office of Naval Research
- Purdue University
- United States Department of Energy