Directed Evolution of an Improved Aminoacyl‐tRNA Synthetase for Incorporation of L‐3,4‐Dihydroxyphenylalanine (L‐DOPA)
Abstract
The catechol group of 3,4‐dihydroxyphenylalanine (L‐DOPA) derived from L‐tyrosine oxidation is a key post‐translational modification (PTM) in many protein biomaterials and has potential as a bioorthogonal handle for precision protein conjugation applications such as antibody–drug conjugates. Despite this potential, indiscriminate enzymatic modification of exposed tyrosine residues or complete replacement of tyrosine using auxotrophic hosts remains the preferred method of introducing the catechol moiety into proteins, which precludes many protein engineering applications. We have developed new orthogonal translation machinery to site‐specifically incorporate L‐DOPA into recombinant proteins and a new fluorescent biosensor to selectively monitor L‐DOPA incorporation in vivo. We show simultaneous biosynthesis and incorporation of L‐DOPA and apply this translation machinery to engineer a novel metalloprotein containing a DOPA‐Fe chromophore.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 24, 2021
- Source ID
- 10.1002/anie.202100579
Entities
People
- Andrew D Ellington
- Dustin R Klein
- Jennifer S. Brodbelt
- Molly S. Blevins
- Ross Thyer
- Simon d’Oelsnitz
Organizations
- Air Force Office of Scientific Research
- Army Research Office
- National Cancer Institute
- National Institute of General Medical Sciences
- Rice University
- Robert A. Welch Foundation
- University of Texas at Austin