Immuno‐Desorption Electrospray Ionization Mass Spectrometry Imaging Identifies Functional Macromolecules by Using Microdroplet‐Cleavable Mass Tags
Abstract
We present immunoassay‐based desorption electrospray ionization mass spectrometry imaging (immuno‐DESI‐MSI) to visualize functional macromolecules such as drug targets and cascade signaling factors. A set of boronic acid mass tags (BMTs) were synthesized to label antibodies as MSI probes. The boronic ester bond is employed to cross‐link the BMT with the galactosamine‐modified antibody. The BMT can be released from its tethered antibody by ultrafast cleavage of the boronic ester bond caused by the acidic condition of sprayed DESI microdroplets containing water. The fluorescent moiety enables the BMT to work in both optical and MS imaging modes. The positively charged quaternary ammonium group enhances the ionization efficiency. The introduction of the boron element also makes mass tags readily identified because of its unique isotope pattern. Immuno‐DESI‐MSI provides an appealing strategy to spatially map macromolecules beyond what can be observed by conventional DESI‐MSI, provided antibodies are available to the targeted molecules of interest.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 24, 2023
- Source ID
- 10.1002/anie.202216969
Entities
People
- Chao Li
- Qingce Zang
- Richard Zare
- Tianhao Zhou
- Xiaowei Song
Organizations
- Air Force Office of Scientific Research
- Fudan University
- National Natural Science Foundation of China
- Peking Union Medical College
- Stanford University
- Tianjin Medical University