Synthesis of Erythrocyte Nanodiscs for Bacterial Toxin Neutralization
Abstract
Nanodiscs are a compelling nanomedicine platform due to their ultrasmall size and distinct disc shape. Current nanodisc formulations are made primarily with synthetic lipid bilayers and proteins. Here, we report a cellular nanodisc made with human red blood cell (RBC) membrane (denoted “RBC‐ND”) and show its effective neutralization against bacterial toxins. In vitro, RBC‐ND neutralizes the hemolytic activity and cytotoxicity caused by purified α‐toxin or complex whole secreted proteins (wSP) from methicillin‐resistant Staphylococcus aureus bacteria. In vivo, RBC‐ND confers significant survival benefits for mice intoxicated with α‐toxin or wSP in both therapeutic and prevention regimens. Moreover, RBC‐ND shows good biocompatibility and biosafety in vivo. Overall, RBC‐ND distinguishes itself by inheriting the biological functions of the source cell membrane for bioactivity. The design strategy of RBC‐ND can be generalized to other types of cell membranes for broad applications.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 20, 2023
- Source ID
- 10.1002/anie.202301566
Entities
People
- Dan Wang
- Ilkoo Noh
- Lei Sun
- Liangfang Zhang
- Ronnie H Fang
- Weiwei Gao
Organizations
- Defense Threat Reduction Agency
- University of California, San Diego