Influence of Sulfur‐Containing Diamino Acid Structure on Covalently Crosslinked Copolypeptide Hydrogels
Abstract
Biologically occurring non‐canonical di‐α‐amino acids were converted into new di‐N‐carboxyanhydride (di‐NCA) monomers in reasonable yields with high purity. Five different di‐NCAs were separately copolymerized with tert‐butyl‐l‐glutamate NCA to obtain covalently crosslinked copolypeptides capable of forming hydrogels with varying crosslinker density. Comparison of hydrogel properties with residue structure revealed that different di‐α‐amino acids were not equivalent in crosslink formation. Notably, l‐cystine was found to produce significantly weaker hydrogels compared to l‐homocystine, l‐cystathionine, and l‐lanthionine, suggesting that l‐cystine may be a sub‐optimal choice of di‐α‐amino acid for preparation of copolypeptide networks. The di‐α‐amino acid crosslinkers also provided different chemical stability, where disulfide crosslinks were readily degraded by reduction, and thioether crosslinks were stable against reduction. This difference in response may provide a means to fine tune the reduction sensitivity of polypeptide biomaterial networks.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 17, 2018
- Source ID
- 10.1002/asia.201801031
Entities
People
- Eric D. Raftery
- Eric G. Gharkhanian
- Jack J. McNamara
- Nicole G. Ricapito
- Timothy J. Deming
Organizations
- Defense Advanced Research Projects Agency
- University of California, Los Angeles