Immobilization of transgenic plant cells towards bioprinting for production of a recombinant biodefense agent
Abstract
Transgenic rice cells (Oryza sativa) producing recombinant butyrylcholinesterase (BChE) as a prophylactic/therapeutic against organophosphate nerve agent poisoning, cocaine toxicity, and neurodegenerative diseases like Alzheimer's were immobilized in a polyethylene glycol‐based hydrogel. The cells were sustained for 14 days in the semi‐solid matrix, undergoing a growth phase from days 0–6, a BChE production phase in sugar‐free medium from days 6–12, and a growth/recovery phase from days 12–14. Throughout this period, the cells maintained similar viability to those in suspension cultures and displayed analogous sugar consumption trends. The rice cells in the hydrogel also produced a significant amount of active BChE, comparable to the levels produced in liquid cultures. A considerable fraction of this BChE was secreted into the media, allowing for easier product separation. To the best of our knowledge, this proof‐of‐concept is the first report of immobilization of recombinant plant cells for continuous production of high‐value heterologous proteins. This work serves as a foundation for further investigation towards plant cell bioprinting and the development of a simple, efficient, robust, modular, and potentially field‐deployable bioreactor system for the manufacture of biologics.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 15, 2021
- Source ID
- 10.1002/biot.202100133
Entities
People
- Anika Varma
- Hawi B. Gemeda
- Jennifer M Knipe
- Karen A McDonald
- Matthew J McNulty
- Somen Nandi
Organizations
- Defense Threat Reduction Agency
- Lawrence Livermore National Laboratory
- University of California