Electrochemical activation of engineered protein switches
Abstract
Engineered protein switches have a large dynamic range, high specificity for the activating ligand, and a modular architecture, and have been explored for a wide range of applications including biosensors and therapeutics. The ability to externally control switch function is important in extending applications for protein switches. We recently demonstrated that the on/off state could be controlled by the redox state of disulfide bonds introduced into the switches at select locations. Here, we demonstrate that an electrochemical signal can be used as an exogenous input to control switch function via reduction of the engineered disulfide bonds. This study suggests that disulfide‐containing protein switch is a potentially useful platform for bioelectronic sensors with remote control of the sensing ability. Biotechnol. Bioeng. 2016;113: 453–456. © 2015 Wiley Periodicals, Inc.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 04, 2015
- Source ID
- 10.1002/bit.25720
Entities
People
- Jay H. Choi
- Marc Ostermeier
- Maya Zayats
- Peter C. Searson
Organizations
- Defense Threat Reduction Agency
- Johns Hopkins University