Direct Intracellular Delivery of Cell‐Impermeable Probes of Protein Glycosylation by Using Nanostraws
Abstract
Bioorthogonal chemistry is an effective tool for elucidating metabolic pathways and measuring cellular activity, yet its use is currently limited by the difficulty of getting probes past the cell membrane and into the cytoplasm, especially if more complex probes are desired. Here we present a simple and minimally perturbative technique to deliver functional probes of glycosylation into cells by using a nanostructured “nanostraw” delivery system. Nanostraws provide direct intracellular access to cells through fluid conduits that remain small enough to minimize cell perturbation. First, we demonstrate that our platform can deliver an unmodified azidosugar, N‐azidoacetylmannosamine, into cells with similar effectiveness to a chemical modification strategy (peracetylation). We then show that the nanostraw platform enables direct delivery of an azidosugar modified with a charged uridine diphosphate group (UDP) that prevents intracellular penetration, thereby bypassing multiple enzymatic processing steps. By effectively removing the requirement for cell permeability from the probe, the nanostraws expand the toolbox of bioorthogonal probes that can be used to study biological processes on a single, easy‐to‐use platform.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 14, 2017
- Source ID
- 10.1002/cbic.201600689
Entities
People
- Alexander M. Xu
- Derek S. Wang
- Nicholas A Melosh
- Peyton Shieh
- Yuhong Cao
Organizations
- California Institute of Technology
- National Science Foundation
- Stanford University
- United States Department of Defense