Symmetry‐Directed Self‐Assembly of a Tetrahedral Protein Cage Mediated by de Novo‐Designed Coiled Coils
Abstract
The organization of proteins into new hierarchical forms is an important challenge in synthetic biology. However, engineering new interactions between protein subunits is technically challenging and typically requires extensive redesign of protein–protein interfaces. We have developed a conceptually simple approach, based on symmetry principles, that uses short coiled‐coil domains to assemble proteins into higher‐order structures. Here, we demonstrate the assembly of a trimeric enzyme into a well‐defined tetrahedral cage. This was achieved by genetically fusing a trimeric coiled‐coil domain to its C terminus through a flexible polyglycine linker sequence. The linker length and coiled‐coil strength were the only parameters that needed to be optimized to obtain a high yield of correctly assembled protein cages.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 29, 2017
- Source ID
- 10.1002/cbic.201700406
Entities
People
- Aaron Sciore
- Ajitha S. Cristie‐david
- Brandon T. Ruotolo
- James C. A. Bardwell
- Joseph D. Eschweiler
- Min Su
- Neil Marsh
- Philipp Koldewey
- Somayesadat Badieyan
Organizations
- Army Research Office
- Howard Hughes Medical Institute
- University of Michigan