2D Enzyme Cascade Network with Efficient Substrate Channeling by Swinging Arms
Abstract
In living cells, compartmentalized or membrane‐associated enzymes are often assembled into large networks to cooperatively catalyze cascade reaction pathways essential for cellular metabolism. Here, we report the assembly of an artificial 2D enzyme network of two cascade enzymes—glucose‐6‐phosphate dehydrogenase (G6PDH) and lactate dehydrogenase (LDH)—on a wireframe DNA origami template. Swinging arms were used to facilitate the transport of the redox intermediate of NAD+/NADH between enzyme pairs on the array. The assemblies of 2D enzyme networks were characterized by gel electrophoresis and visualized by atomic force microscopy (AFM). The spatial arrangements of multiple enzyme pairs were optimized to facilitate efficient substrate channeling by exploiting the programmability of DNA origami to manipulate the key parameters of swinging arm length and stoichiometry. Compared with a single enzyme pair, the 2D organized enzyme systems exhibited higher reaction efficiency due to the promoted transfer of intermediates within the network.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 04, 2018
- Source ID
- 10.1002/cbic.201700613
Entities
People
- Hao Yan
- Jinglin Fu
- Minghui Liu
- Shaun Wootten
- Xiaodong Qi
- Yan Liu
- Yuhe Renee Yang
Organizations
- Arizona State University
- National Institutes of Health
- Rutgers University
- Rutgers University–Camden
- United States Army Research Laboratory