Self‐Assembly of Hybrid Peptide−DNA Nanostructures using Homotrimeric Coiled‐Coil/Nucleic Acid Building Blocks
Abstract
Peptides and DNA are two of the most commonly used self‐assembling biological molecules for the construction of nanomaterials. However, there are only a few examples that combine these two self‐assembly motifs as key structural elements in a nanostructure. We report on the synthesis of a peptide−DNA conjugate that self‐assembles into a stable homotrimer based on the coiled‐coil motif. The hybrid peptide−DNA trimer was then used as a novel three‐way junction to link together either small DNA tile nanostructures, or to close up a triangular wireframe DNA structure. The resulting nanostructures were characterized by atomic force microscopy, and compared with a scrambled, non‐assembling peptide as a control. These hybrid nanostructures enable the integration of peptide motifs and potentially bio‐functionality with DNA nanostructures, and open the door to novel nano‐materials that have the advantages of both molecules.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 09, 2023
- Source ID
- 10.1002/cbic.202300223
Entities
People
- Alex Buchberger
- Chad R Simmons
- Md Al‐amin
- Nicholas Stephanopoulos
Organizations
- Air Force Office of Scientific Research
- Arizona State University
- National Institutes of Health