Enzymatic Bioelectrocatalysis for Enzymology Applications
Abstract
Enzymatic biosensors are commonly used to evaluate the concentration of individual analytes in samples (e. g., glucose in blood, lactate in sweat, etc.), where the working electrode utilizes an enzyme (a “bioelectrode”) as the chemically selective agent for electrochemical analyses. However, those same enzymatic bioelectrodes can be used for the analytical characterization of enzymes as a new biophysical characterization tool for enzymologists. This Minireview will detail recent work focused on utilizing enzymatic bioelectrocataysis for enzymology applications. Specifically, it will talk about the use of enzymatic bioelectrodes for studying kinetics, inhibition, transport, and thermodynamics. It will also compare and contrast electrochemical techniques with spectrophotometric techniques for enzymology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 06, 2020
- Source ID
- 10.1002/celc.202000239
Entities
People
- Matthew J. Kummer
- Shelley D. Minteer
Organizations
- Air Force Office of Scientific Research
- University of Utah