Morphological principles of neuronal mitochondria
Abstract
In the highly dynamic metabolic landscape of a neuron, mitochondrial membrane architectures can provide critical insight into the unique energy balance of the cell. Current theoretical calculations of functional outputs like adenosine triphosphate and heat often represent mitochondria as idealized geometries, and therefore, can miscalculate the metabolic fluxes. To analyze mitochondrial morphology in neurons of mouse cerebellum neuropil, 3D tracings of complete synaptic and axonal mitochondria were constructed using a database of serial transmission electron microscopy (TEM) tomography images and converted to watertight meshes with minimal distortion of the original microscopy volumes with a granularity of 1.64 nanometer isotropic voxels. The resulting in‐silico representations were subsequently quantified by differential geometry methods in terms of the mean and Gaussian curvatures, surface areas, volumes, and membrane motifs, all of which can alter the metabolic output of the organelle. Finally, we identify structural motifs present across this population of mitochondria, which may contribute to future modeling studies of mitochondrial physiology and metabolism in neurons.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 01, 2021
- Source ID
- 10.1002/cne.25254
Entities
People
- Adam Husar
- Alexander Skupin
- Christopher T Lee
- Donald J. Spencer
- Emily Liu
- Eric A. Bushong
- Guadalupe C Garcia
- Guy Perkins
- Mark H Ellisman
- Padmini Rangamani
- Priya Khandelwal
- Rachel Mendelsohn
- Sébastien Phan
- Terrence J. Sejnowski
- Thomas M. Bartol
Organizations
- Air Force Office of Scientific Research
- Blueprint for Neuroscience Research
- Office of Naval Research
- Salk Institute for Biological Studies
- University of California, San Diego
- University of Luxembourg