Energy‐Resolved Mass Spectrometry as a Tool for Identification of Lignin Depolymerization Products

Abstract

Lignin is the largest source of bio‐based aromatic compounds in nature, and its valorization is essential to the sustainability of lignocellulosic biorefining. Characterizing lignin‐derived compounds remains challenging due to the heterogeneity of this biopolymer. Tandem mass spectrometry is a promising tool for lignin structural analytics, as fragmentation patterns of model compounds can be extrapolated to identify characteristic moieties in complex samples. This work extended previous resonance excitation‐type collision‐induced dissociation (CID) methods that identified lignin oligomers containing β–O–4, β–5, and β–β bonds, to also identify characteristics of 5–5, β–1, and 4–O–5 dimers, enabled by quadrupole time‐of‐flight (QTOF) CID with energy‐resolved mass spectrometry (ERMS). Overall, QTOF‐ERMS offers in‐depth structural information and could ultimately contribute to tools for high‐throughput lignin dimer identification.

Document Details

Document Type
Pub Defense Publication
Publication Date
Nov 16, 2022
Source ID
10.1002/cssc.202201441

Entities

People

  • Brenna A. Black
  • Gregg T. Beckham
  • Heather B Mayes
  • John Ralph
  • Kris Morreel
  • Rui Katahira
  • Xueming Dong
  • Yanding Li

Organizations

  • Bioenergy Technologies Office
  • Great Lakes Bioenergy Research Center
  • National Renewable Energy Laboratory
  • Oak Ridge National Laboratory
  • Office of Energy Efficiency and Renewable Energy
  • United States Department of Energy
  • University of Wisconsin–Madison

Tags

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Forest Ecology
  • Molecular and Cellular Biochemistry