High‐throughput precision measurement of subcellular localization in single cells
Abstract
To quantify visual and spatial information in single cells with a throughput of thousands of cells per second, we developed Subcellular Localization Assay (SLA). This adaptation of Proximity Ligation Assay expands the capabilities of flow cytometry to include data relating to localization of proteins to and within organelles. We used SLA to detect the nuclear import of transcription factors across cell subsets in complex samples. We further measured intranuclear re‐localization of target proteins across the cell cycle and upon DNA damage induction. SLA combines multiple single‐cell methods to bring about a new dimension of inquiry and analysis in complex cell populations. © 2017 International Society for Advancement of Cytometry
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 17, 2017
- Source ID
- 10.1002/cyto.a.23054
Entities
People
- Amanda R. Groziak
- Andreas P. Frei
- Felice A. Bava
- Garry P. Nolan
- Jake E. Batchelder
- Julie M. Yu
- Pier Federico Gherardini
- Samuel C. Kimmey
- Tyler J. Burns
- Veronica D. Gonzalez
- Wendy J. Fantl
- Yuki Yoshiyasu
Organizations
- Barnard College
- California Institute for Regenerative Medicine
- Chiron Corporation
- Congressionally Directed Medical Research Programs
- Cornell University
- Entertainment Industry Foundation
- Food and Drug Administration
- Gates Foundation
- Gilead Sciences
- National Institutes of Health
- Pfizer
- Stanford University
- University of Texas at Austin