Validation of a nutria (Myocastor coypus) environmental DNA assay highlights considerations for sampling methodology
Abstract
Nutria (Myocastor coypus) is a semiaquatic rodent species that is invasive across multiple regions within the United States. Here, we evaluated a qPCR assay previously described for use in Japan for application across invasive populations in the United States. We also compared two environmental DNA sampling methodologies for this assay: field filtration of large volumes of water passed through filters versus direct sampling of small volumes of water. We validated assay specificity, generality, and sensitivity, compared assay performance between two independent laboratories, and successfully tested the assay in situ on a known wild population. The filtration method required fewer samples for environmental DNA detection than direct sampling, but the choice of methods should be assessed based on specific field conditions and time and budget considerations. Our extensive assay validation and comparison across laboratories suggest that the assay is ready to be applied in environmental DNA monitoring of nutria throughout the United States.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 01, 2023
- Source ID
- 10.1002/edn3.412
Entities
People
- Anna M Mangan
- Antoinette J. Piaggio
- Ian H. Plummer
- John A Kronenberger
- Taylor M Wilcox
Organizations
- Environmental Security Technology Certification Program
- National Wildlife Research Center
- Rocky Mountain Research Station
- Wildlife Services