Transcription factor YY1 can control AID‐mediated mutagenesis in mice
Abstract
Activation‐induced cytidine deminase (AID) is crucial for controlling the immunoglobulin (Ig) diversification processes of somatic hypermutation (SHM) and class switch recombination (CSR). AID initiates these processes by deamination of cytosine, ultimately resulting in mutations or double strand DNA breaks needed for SHM and CSR. Levels of AID control mutation rates, and off‐target non‐Ig gene mutations can contribute to lymphomagenesis. Therefore, factors that control AID levels in the nucleus can regulate SHM and CSR, and may contribute to disease. We previously showed that transcription factor YY1 can regulate the level of AID in the nucleus and Ig CSR. Therefore, we hypothesized that conditional knock‐out of YY1 would lead to reduction in AID localization at the Ig locus, and reduced AID‐mediated mutations. Using mice that overexpress AID (IgκAID yy1f/f) or that express normal AID levels (yy1f/f), we found that conditional knock‐out of YY1 results in reduced AID nuclear levels, reduced localization of AID to the Sμ switch region, and reduced AID‐mediated mutations. We find that the mechanism of YY1 control of AID nuclear accumulation is likely due to YY1‐AID physical interaction which blocks AID ubiquitination.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 14, 2017
- Source ID
- 10.1002/eji.201747065
Entities
People
- Arindam Basu
- Boris Tichy
- Hans-Martin Jäck
- Jitka Malcikova
- Kristina Zaprazna
- Lenka Radova
- Nikola Tom
- Sarka Pospisilova
- Suchita Hodawadekar
- Vibha Jha
Organizations
- Central European Institute of Technology
- Masaryk University
- National Institute of Allergy and Infectious Diseases
- National Institute of General Medical Sciences
- United States Army
- University of Pennsylvania