ITP of lanthanides in microfluidic PMMA chip
Abstract
An ITP separation of eight lanthanides on a serpentine PMMA microchip with a tee junction and a 230‐mm‐long serpentine channel is described. The cover of the PMMA chip is 175 μm thick so that a C4D in microchip mode can be used to detect the lanthanides as they migrate through the microchannel. Acetate and α‐hydroxyisobutyric acid are used as complexing agents to increase the electrophoretic mobility difference between the lanthanides. Eight lanthanides are concentrated within ∼ 6 min by ITP in the microchip using 10 mM ammonium acetate at pH 4.5 as the leading electrolyte and 10 mM acetic acid at ∼ pH 3.0 as the terminating electrolyte. In addition, a 2D numerical simulation of the lanthanides undergoing ITP in the microchip is compared with experimental results using COMSOL Multiphysics v4.3a.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 27, 2013
- Source ID
- 10.1002/elps.201300382
Entities
People
- Bingwen Liu
- Cornelius F Ivory
- Danny Bottenus
- Sue B. Clark
- Yongzheng Cong
Organizations
- Defense Threat Reduction Agency
- National Science Foundation
- United States Department of Energy
- United States Department of Homeland Security
- Washington State University