Accurate color imaging of pathology slides using holography and absorbance spectrum estimation of histochemical stains
Abstract
Holographic microscopy presents challenges for color reproduction due to the usage of narrow‐band illumination sources, which especially impacts the imaging of stained pathology slides for clinical diagnoses. Here, an accurate color holographic microscopy framework using absorbance spectrum estimation is presented. This method uses multispectral holographic images acquired and reconstructed at a small number (e.g., three to six) of wavelengths, estimates the absorbance spectrum of the sample, and projects it onto a color tristimulus. Using this method, the wavelength selection is optimized to holographically image 25 pathology slide samples with different tissue and stain combinations to significantly reduce color errors in the final reconstructed images. The results can be used as a practical guide for various imaging applications and, in particular, to correct color distortions in holographic imaging of pathology samples spanning different dyes and tissue types.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 19, 2018
- Source ID
- 10.1002/jbio.201800335
Entities
People
- Alborz Feizi
- Aydoğan Özcan
- Da Teng
- Tairan Liu
- Yair Rivenson
- Yibo Zhang
- Yichen Wu
- Yinxu Bian
- Yujia Huang
Organizations
- Army Research Office
- California NanoSystems Institute
- Department of Surgery
- Howard Hughes Medical Institute
- Mary Kay Foundation
- National Institutes of Health
- National Science Foundation
- Yale University