Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues
Abstract
Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 19, 2019
- Source ID
- 10.1002/jbio.201800449
Entities
People
- Anita Mahadevan‐jansen
- Christine M. O'Brien
- David M. Aronoff
- Jennifer A. Gaddy
- Oscar D Ayala
- Ryan S. Doster
- Shannon D. Manning
Organizations
- Michigan State University
- National Cancer Institute
- National Center for Advancing Translational Sciences
- National Institutes of Health
- United States Department of Defense
- United States Department of Veterans Affairs
- Vanderbilt University
- Vanderbilt University Medical Center