Low‐photobleaching line‐scanning confocal microscopy using dual inclined beams
Abstract
Confocal microscopy is an indispensable tool for biological imaging due to its high resolution and optical sectioning capability. However, its slow imaging speed and severe photobleaching have largely prevented further applications. Here, we present dual inclined beam line‐scanning (LS) confocal microscopy. The reduced excitation intensity of our imaging method enabled a 2‐fold longer observation time of fluorescence compared to traditional LS microscopy while maintaining a good sectioning capability and single‐molecule sensitivity. We characterized the performance of our method and applied it to subcellular imaging and three‐dimensional single‐molecule RNA imaging in mammalian cells.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 14, 2019
- Source ID
- 10.1002/jbio.201900075
Entities
People
- Jialei Tang
- Kyu Young Han
Organizations
- Defense Advanced Research Projects Agency
- National Science Foundation Directorate for Engineering
- University of Central Florida