Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination
Abstract
Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high‐mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pneumonia. In this study, we analyzed key parameters for the viability of this treatment, including the selectivity of the photodynamic response for pathogens over host cells. Our results showed that PDI can induce killing of Staphylococcus aureus (of up to 4.18 log for the strain Xen29 and 3.62 log for Xen36) under conditions where little or no toxicity for host cells is observed. We validated pulmonary delivery of the photosensitizer and light in mice, using photobleaching as an indicator, and demonstrated preservation of healthy tissues as evidence of the safety of the protocol. Overall, PDI displays low toxicity on host tissues, making it a promising tool for treatment of pneumonias caused by S. aureus and other important pathogens.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 12, 2020
- Source ID
- 10.1002/jbio.202000176
Entities
People
- Cristina Kurachi
- Giulia Kassab
- Jace A. Willis
- Jeffrey D. Cirillo
- Madeleine G. Moule
- Vanderlei S. Bagnato
- Vladislav V Yakovlev
- Vsevolod Cheburkanov
Organizations
- Air Force Office of Scientific Research
- Cancer Prevention and Research Institute of Texas
- Defense Advanced Research Projects Agency
- Division of Biological Infrastructure
- Division of Civil, Mechanical & Manufacturing Innovation
- Division of Electrical, Communications & Cyber Systems
- National Council for Scientific and Technological Development
- National Institute of Allergy and Infectious Diseases
- National Institute of General Medical Sciences
- Office of Naval Research
- São Paulo Research Foundation
- United States Army Research Laboratory
- University of São Paulo