Imaging approaches for monitoring three‐dimensional cell and tissue culture systems
Abstract
The past decade has seen an increasing demand for more complex, reproducible and physiologically relevant tissue cultures that can mimic the structural and biological features of living tissues. Monitoring the viability, development and responses of such tissues in real‐time are challenging due to the complexities of cell culture physical characteristics and the environments in which these cultures need to be maintained in. Significant developments in optics, such as optical manipulation, improved detection and data analysis, have made optical imaging a preferred choice for many three‐dimensional (3D) cell culture monitoring applications. The aim of this review is to discuss the challenges associated with imaging and monitoring 3D tissues and cell culture, and highlight topical label‐free imaging tools that enable bioengineers and biophysicists to non‐invasively characterise engineered living tissues.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 24, 2022
- Source ID
- 10.1002/jbio.202100380
Entities
People
- Emma T. Gill
- Geva Hilzenrat
- Sally L. McArthur
Organizations
- Air Force Office of Scientific Research
- Canada Excellence Research Chairs
- Commonwealth Scientific and Industrial Research Organisation
- Swinburne University of Technology