Laser‐induced shockwave paired with FRET: A method to study cell signaling
Abstract
Cells within the body are subject to various forces; however, the details concerning the way in which cells respond to mechanical stimuli are not well understood. We demonstrate that laser‐induced shockwaves (LIS) combined with biosensors based on fluorescence resonance energy transfer (FRET) is a promising new approach to study biological processes in single live cells. As “proof‐of‐concept,” using a FRET biosensor, we show that in response to LIS, cells release intracellular calcium. With the parameters used, cells retain their morphology and remain viable. LIS combined with FRET permits observation of the cells immediate response to a sudden shear force. Microsc. Res. Tech. 78:195–199, 2015. © 2015 Wiley Periodicals, Inc.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 13, 2015
- Source ID
- 10.1002/jemt.22463
Entities
People
- Daryl Preece
- Derrick Rosales
- Lie Lei
- Linda Shi
- Michael W. Berns
- Nima Khatibzadeh
- Veronica Gomez‐godinez
- Yijia Pan
- Yingxiao Wang
Organizations
- Beckman Laser Institute
- National Science Foundation
- United States Department of Defense
- University of California
- University of California, San Diego