Metastatic prostate cancer‐derived extracellular vesicles facilitate osteoclastogenesis by transferring the CDCP1 protein
Abstract
Bone metastases are still incurable and result in the development of clinical complications and decreased survival for prostate cancer patients. Recently, a number of studies have shown that extracellular vesicles (EVs) play important roles in tumour progression. Here, we show that EVs from metastatic prostate cancer cells promote osteoclast formation in the presence of receptor activator of NF‐κB ligand (RANKL). EV characterization followed by functional siRNA screening identified CUB‐domain containing protein 1 (CDCP1), a transmembrane protein, as an inducer of osteoclastogenesis. Additionally, CDCP1 expression on plasma‐derived EVs was upregulated in bone metastatic prostate cancer patients. Our findings elucidate the effect of EVs from metastatic prostate cancer cells on osteoclast formation, which is promoted by CDCP1 located on EVs. Furthermore, our data suggested that CDCP1 expression on EVs might be useful to detect bone metastasis of prostate cancer.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 01, 2023
- Source ID
- 10.1002/jev2.12312
Entities
People
- Carolina Soekmadji
- Fumihiko Urabe
- Kagenori Ito
- Kurataka Otsuka
- Nobuyoshi Kosaka
- Shin Egawa
- Takahiro Kimura
- Takahiro Ochiya
- Yusuke Yamamoto
Organizations
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science
- Japanese Foundation for Prostate Research
- Japanese Urological Association
- Jikei University School of Medicine
- Kewpie Corporation
- National Cancer Center
- Tokyo Medical University
- United States Department of Defense
- University of Queensland