Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis
Abstract
Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12‐week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, pJournal of Orthopaedic Research published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 33:1142–1151, 2015.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 14, 2015
- Source ID
- 10.1002/jor.22899
Entities
People
- Andrew P. Breidenbach
- Chia‐feng Liu
- Chris Wylie
- David L. Butler
- David W Rowe
- Han Liu
- Jason T. Shearn
- Karl E. Kadler
- Lindsey Aschbacher‐smith
- Marepalli Rao
- Nathaniel A Dyment
- Rulang Jiang
- Yinhui Lu
Organizations
- Cincinnati Children's Hospital Medical Center
- Cleveland Clinic
- National Institutes of Health
- United States Department of Defense
- University of Cincinnati
- University of Connecticut
- University of Manchester