In vitro and in vivo assessment of extended duration cathodic voltage‐controlled electrical stimulation for treatment of orthopedic implant‐associated infections
Abstract
Effective treatment of orthopedic implant‐associated infections (IAIs) remains a clinical challenge. The in vitro and in vivo studies presented herein evaluated the antimicrobial effects of applying cathodic voltage‐controlled electrical stimulation (CVCES) to titanium implants inoculated with preformed bacterial biofilms of methicillin‐resistant Staphylococcus aureus (MRSA). The in vitro studies showed that combining vancomycin therapy (500 µg/mL) with application of CVCES at −1.75 V (all voltages are with respect to Ag/AgCl unless otherwise stated) for 24 h resulted in 99.98% reduction in the coupon‐associated MRSA colony‐forming units (CFUs) (3.38 × 103 vs. 2.14 × 107CFU/mL, p 4 vs. 1.26 × 108CFU/mL, p 1 vs. 1.2 × 106 CFU/mL, p 1 vs. 4.48 × 106 CFU/mL, p < 0.003) as compared with the untreated control animals. Importantly, the combined 24 h CVCES and antibiotic treatments resulted in no implant‐associated MRSA CFU enumerated in 83% of the animals (five out of six animals) and no bone‐associated MRSA CFU enumerated in 50% of the animals (three out of six animals). Overall, the outcomes of this study have shown that extended duration CVCES therapy is an effective adjunctive therapy to eradicate IAIs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 05, 2023
- Source ID
- 10.1002/jor.25625
Entities
People
- Albert Titus
- Caelen Clark
- Eric Mcdermott
- Gowtham Mohanraj
- Kyle Weeks
- Mark T Ehrensberger
- Menachem Tobias
- Thomas Duquin
Organizations
- Office of Naval Research
- University at Buffalo